뇌질환의 혁신, 뉴로벤티(NeuroVenti)

R&D SERVICES

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From early-stage discovery to clinical candidate

Animal models for neurological disorders

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Depression 우울증
  • Acute restraint stress, Chronic mild stress, Maternal deprivation
  • Corticosterone/LPS-injection
AD/Dementia 알츠하이머병
  • Genetic: 5xFAD Tg mice
  • Aβ/scopolamine/LPS injection, DM-induced dementia model, BCCAO
PD 파킨슨병
  • 6-OHDA/
    rotenone/MPTP
    injection
  • α-synuclein overexpression
  • A53T Tg mice
Schizophrenia 조현병
  • MK-801/ PCP/
    amphetamine/
    Poly(I:C)-injection
  • Social stress model (social isolation, social defeat)
ASD 자폐증
  • Genetic : CNTNAP2 KO, Fmr1 KO mice, AGMAT Tg mice
  • Chemical : VPA/Poly(I:C)-injection
ADHD ADHD
  • Genetic: SHR, Fmr1 KO
  • Chemical: EtOH/MK-801-injection
Epilepsy 뇌전증
  • Electricity induced model (MES)
  • Chemical induced model (PTZ, KA, Bic)
  • Kindling
  • Febrile sz
  • Sz-prone animals
Stroke 뇌졸증
  • Photothrombosis-induced model, MCAO
  • ET-1/ Hemin/ autologous blood injection

In vivo efficacy evaluation platform

AI-based 3D automated behavior analysis system.

3D Behavior Analysis Observation Equipment
Multi-object simultaneous analysis equipment, researcher simultaneous observation equipment

Ethovision Analysis System

Ethovision Analysis System
Behavior domain Evaluation method Behavior domain Evaluation method
Sociability & communication
  • Three chamber test
  • Home-cage social test
  • Ultra sonic vocalization test
Learning & memory
  • Barnes maze test
  • Y-maze test
  • Novel object recognition test
  • Passive avoidance test
  • Morris water maze test
Depression & anxiety
  • Forced swim test
  • Tail suspension test
  • Sucrose preference test
  • Open field test
  • Novelty suppressed feeding test
  • Elevated plus maze test
  • Light dark box test
Motor function
  • Open field test
  • Rotarod test
  • Footprint test
  • Beam walking test
  • Grip strength test
  • Grid hanging test
  • Pole test
  • Cylinder test
  • Grid waking test
  • Rotation test
Repetitive behavior
  • Self-grooming test
  • Marble-burying test
  • Nest building test
Impulsivity
  • Cliff avoidance test
  • 5-choice serial reaction time test
  • Delayed discounting test

Verification and Evaluation of Side Effects of CNS

Prepulse inhibition test

Prepulse inhibition test

CPP/SA test

CPP/SA test

Sleep disturbance

Sleep disturbance

Seizure

Growth retardation/FOB
Evaluation Domain Evaluation method Evaluation Domain Evaluation method
Dependence
  • Evaluation of conditional place preferences
  • Self-administered test
Sleep disorder
  • Sleep test (measurement of sleep time and sleep duration)
  • Brainwave measurement(EEG telemetry)
Sensory exercise gating
  • Antecedent stimulation suppression test
Convulsions
  • Chemical induced seizure
  • Electricity induced seizure
  • Threshold
  • Brainwave measurement(EEG telemetry)

In vitro efficacy evaluation & MOA research platform

Test area Evaluation method
Primary cell culture model
  • Primary neuron/astrocyte/microglia culture
  • Neural precursor cell culture
  • Organotypic brain slice culture
Cell lines
  • HT22, HN3, Neuro2A, N27, SH-SY5Y, PC12, C6 glioma, BV-2, bEnd.3 cell
Neuronal cell death & neurite outgrowth
  • Incucyte zoom live cell imaging system
Mitochondrial function
  • OCR : Seahorse XF Cell Mito Stress Analyzer system
  • Fusion/Fission : ICC (Drp1 and PGC1alpha)
  • Biogenesis : TIMM23, TOMM20, SDHA, COX4, PDK4, SMAC expression, mtDNA
  • ROS : mitoSOX, Mitotracker, TMRM
Neurotransmitter change
  • Fast scan cyclic voltammetry
  • Functional reuptake assay (NET, SERT, DAT)
Synaptogenesis and synaptic change
  • Morphology: EM, Confocal Imaging
  • Alternation of synapse protein: WB, IHC/ICC (PSD95, synaptophysin, synapsin, SNARE etc)
Neurogenesis and differentiation
  • Proliferation: Immunostaining(Ki-67, BrdU, Nestin, doublecortin)
  • Differentiation: tPA/PAI-1/MMPs zymography system, Immunostaining(Tuj-1, NCAM, BDNF)
BBB permeability & breakdown
  • Immunostaining: Tight junction molecules, GFAP, vWF, NG2, PDGF
  • BBB integrity: Evans blue dye, IgG extravasation, brain edema
Antioxidant and neuroinflammation
  • Antioxidant enzyme: HO-1, GSH, NQO1
  • ROS measurement: DHE, DCFH2DA, MDA, 4-HNE
  • Expression and release of Inflammatory cytokine: RT-PCR, WB, ELISA
Discovery of New target
  • Microarray, PCR Array, Real time-PCR

Adverse effect & addiction verification platform

Test area Evaluation method Test drug
Receptor binding assay
  • Dopamine receptor binding assay
  • Dopamine reuptake assay
  • GABA receptor binding assay
  • Chloride ion uptake test
  • Norepinephrine & serotonin reuptake assay
  • Opioid receptor binding assay (μ, δ, κ-receptor)
  • Cannabinoid (CB1, CB2) receptor binding assay
  • Stimulants
  • Sedative hypnotics
  • General anesthetics
  • Anti-anxiety medications & antidepressants
  • Narcotic analgesics
  • Cannabinoid derivatives
Mental dependence
  • Conditioned place preference test
  • Self administration test
Physical dependence
  • Spontaneous movement(Locomotor test, Prepulse inhibition test, Treadmill test)
  • Convulsion reaction
  • leap test (Cylinder test)
  • Climbing test (Dopamine)
  • Head twitch test (Serotonin)
  • Withdrawal behavior(Rotarod test, Open field test, Elevated plus maze test)
Withdrawal symptom
  • Anxiety (Elevated plus maze test)
  • Cognition (Novel object recognition test)
  • Depressive behavior (Forced swim test)
  • Sensory-motor gating (Prepulse inhibition test)
Drug discrimination
  • Drug discrimination test
Sleep disturbance
  • Pentobarbital-induced sleep model
  • Caffeine-induced sleep deprivation model
  • REM sleep deprivation model
  • EEG
Responsiveness
  • Anhedonia (Sucrose preference test)
Seizure
  • Seizure scoring
  • Electrically-induced model
  • Chemically-induced model
  • EEG


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